Solving unique analytical challenges in mRNA-LNP bioprocessing with precise measurement methods

Tuesday 08:00 PDT / 11:00 EDT / 15:00 GMT / 16:00 CET

Sponsor

Solving unique analytical challenges in mRNA-LNP bioprocessing with precise measurement methods

Determining mRNA concentration is critical for the development and manufacture of effective mRNA therapeutics - but ensuring accurate measurement poses a number of unique analytical challenges. This webinar will explore the practical application of advanced spectroscopic techniques for measuring mRNA concentration, particularly when encapsulated in LNPS. Key challenges addressed will include:

Sample measurement without dilution: high concentrations of encapsulated mRNA will saturate traditional spectrophotometers, requiring the user to dilute the sample
Concentration measurement in under one minute: Other analytical techniques for measuring encapsulated mRNA, such as Ribogreen or HPLC, can take several hours
More precise mRNA measurement: Multiple measurements of the same sample have been validated to be within 2% RSD, while other techniques can vary up to 10%.

Gain a detailed understanding of methodology applied, specific challenges encountered, and how these techniques can help to enhance the reliability of mRNA manufacturing. Additionally, potential future advancements in spectroscopic technologies for enhancing mRNA therapeutic development will be explored.

Attend this webinar to:

Explore the specific analytical challenges posed by mRNA-LNP therapies
Understand the role spectroscopy approaches can play in achieving precise mRNA concentration measurements, and how to improve upon existing methodologies
Review the development of a method that has been validated to address UV light scattering caused by LNP encapsulation
Assess how effective measurement techniques influence the quality and efficiency of biopharmaceutical manufacturing

Nigel Herbert

Senior Bioanalytics Applications Specialist at Repligen

Nigel Herbert stands as a dedicated advocate for our customers' success. With a Bachelor of Science degree in Biomaterials Engineering and a minor in Chemistry from Alfred University, Nigel brings a wealth of skills to his role as a Senior Bioanalytics Applications Specialist at Repligen.

Since joining our team in 2019, Nigel has been on the forefront of advancing our customers' analytical methods. He specializes in UV-Vis spectroscopy, analytical processes, and method validation, offering invaluable support to ensure the highest standards of precision and reliability.

One of Nigel's remarkable achievements is his collaborative spirit, resulting in numerous published application notes. Through close partnerships with our customers, he has made significant strides in solving complex challenges and driving innovation in the biotechnology field. Nigel Herbert's unwavering commitment to enhancing your analytical capabilities exemplifies Repligen's dedication to your success.